Iago's Motives In Othello

Ruth Frenkel MIC 220 Challenge questions set #2 Due April 3, 2002 SID# 601-50-5380 1.2.          in that location be a multitude of reasons that the Escherichia coli did non produce the interferon after existence transform. First, the E. coli cells might non move over accepted the plasmid DNA during the displacement process. If the electroporation was use, the potency might have been too naughty and irreversibly damaged the cell membrane, thereby rendering the cells incompetent. Conversely, the voltage used might have been too low and not have induced micropores in the cell membrane heavy(a) ample for the uptake of foreign DNA. For E. coli the voltage hustle for no-hit displacement by electroporation is 250 to 4,000 V/cm. Voltages above or down the stairs this range willinging not result in transformed cells, and so will not expressage interferon. Another news report for the neediness of interferon production is that the plasmid did not contain a natural promoter upstream of the inserted interferon component. Without a immobile promoter, the E. colis RNA polymerase will not bind to the recombinant plasmid and thus will not transcribe the interferon constituent. On the other hand, if there was a arduous promoter, the protein could have been over-transcribed and the host cells machinery would be overloaded, resulting in inadequate bearing of the interferon.

Yet another reason for the absence of interferon expression is that the inserted gene fragment did not have a leader sequence, also known as a type peptide. These sequences are typically located at the N-terminus of a protein and facilitate the transport of express prote ins into the periplasm. This exportation ren! ders the expressed foreign protein more stable than if it peace in the E. colis cytol. If the interferon gene was not attached to a star sign peptide, then it may have been degraded in the cytol by endogenous exonucleases. Also, since interferon is... If you want to get a near essay, order it on our website: OrderEssay.net

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